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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/24287

Title: Delivery of Helper-dependent Adenoviral Vectors to the Subretinal Space of Mice
Authors: Wu, Linda
Advisor: Hu, Jim
Department: Laboratory Medicine and Pathobiology
Keywords: eye gene therapy
viral vectors
helper dependent adenovirus
subretinal injections
Issue Date: 7-Apr-2010
Abstract: The helper-dependent adenoviral (HD-Ad) vector is the latest generation of Ad vectors. It ameliorates the vector-associated immunogenic problems with increased capacity for carrying DNA because all viral coding genes are removed. I hypothesize that HD-Ad vectors can be effective vehicles for retinal gene delivery. The objectives of this study are to determine if HD-Ad vectors can deliver reporter genes, GFP or lacZ, driven by a CMV or a MOPS promoter, into specific retinal layers. Subretinal injections were performed and eyes removed at time points from 1 week to 3 months, processed for fluorescent microscopy, X-gal staining, and H&E staining. Transgene expression was detected for at least 3 months. A dose dependent relationship was revealed between the level of transgene expression and viral vector dose. Distinctively, the MOPS promoter drove photoreceptor cell specific expression. Notably, no sign of inflammation or tissue toxicity was detected, demonstrating the benefits of the HD-Ad vector.
URI: http://hdl.handle.net/1807/24287
Appears in Collections:Master
Department of Laboratory Medicine and Pathobiology - Master theses

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