test Browse by Author Names Browse by Titles of Works Browse by Subjects of Works Browse by Issue Dates of Works

Advanced Search
& Collections
Issue Date   
Sign on to:   
Receive email
My Account
authorized users
Edit Profile   
About T-Space   

T-Space at The University of Toronto Libraries >
School of Graduate Studies - Theses >
Doctoral >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/24311

Title: Design and Devlopment of a Biostretch Apparatus for Tissue Engineering
Authors: Pang, Qiming
Advisor: Zu, Jean W.
Department: Mechanical and Industrial Engineering
Issue Date: 13-Apr-2010
Abstract: Tissue engineering has emerged as a promising approach to repair, replace or regenerate damaged tissues using tissue constructs created in vitro. The standard procedure of the strategy to create a functional tissue is to seed cells on a 3-D biodegradable and biocompatible scaffold, to grow them under precisely controlled culture conditions provided by a bioreactor system, and to deliver the matured construct into the patient’s body to induce and direct the growth of the new and healthy tissue. In this thesis, a novel bioreactor system is designed and developed, which can provide uniaxial cyclic stretch to the tissue patch during culture process. The biostretch apparatus employs non-contact electromagnetic force to cyclically stretch a cell-seeded three-dimensional scaffold. The non-contact driving force and the specially designed mount allow researchers to use standard Petri dishes and commercially available CO2 incubators to culture an engineered tissue patch with precisely controlled strain. The device greatly simplifies the procedure to deliver mechanical stimulation during engineering a tissue patch. Since the applied mechanical stimulus is generated by a magnetic force, the engineered tissue construct is not only affected by a mechanical force, but also exposed to a magnetic field. Thus, the effects of the time-varying magnetic field during the culture process are investigated. The flux density of the field is modeled by COMSOL, and verified by a Gaussmeter. In addition, one side effect of using electromagnets, that of a temperature increase, is also investigated. The biomedical experiment results show that neither a weak low frequency magnetic field (0.1T, 1Hz) nor an increase of 1℃ in temperature has a significant effect on the cell culture. The performance of the designed apparatus is verified by the biomedical experiments from the aspects of cell proliferation and reorganization. Moreover, the mechanical parameters (strain distribution, strain rate, and stretch force) provided by the apparatus have also been quantitatively investigated.
URI: http://hdl.handle.net/1807/24311
Appears in Collections:Doctoral
Department of Mechanical & Industrial Engineering - Doctoral theses

Files in This Item:

File Description SizeFormat
Pang_Qiming_200911_PhD_thesis.pdf4.21 MBAdobe PDF

Items in T-Space are protected by copyright, with all rights reserved, unless otherwise indicated.