test Browse by Author Names Browse by Titles of Works Browse by Subjects of Works Browse by Issue Dates of Works
       

Advanced Search
Home   
 
Browse   
Communities
& Collections
  
Issue Date   
Author   
Title   
Subject   
 
Sign on to:   
Receive email
updates
  
My Account
authorized users
  
Edit Profile   
 
Help   
About T-Space   

T-Space at The University of Toronto Libraries >
School of Graduate Studies - Theses >
Master >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/24596

Title: Cellular and Molecular Mechanism Underlying the Effect of Low-magnitude, High-frequency Vibration on Bone
Authors: Lau, Esther Yee Tak
Advisor: You, Lidan
Department: Biomedical Engineering
Keywords: bone
mechanotransduction
osteocyte
osteoclast
mesenchymal stromal cell
whole body vibration
low-magnitude, high-frequency vibration
Issue Date: 27-Jul-2010
Abstract: An emerging non-pharmacological treatment for bone degenerative diseases is whole body vibration (WBV), a mechanical signal composed of low-magnitude, high-frequency (LMHF) vibrations that when applied to bone, have osteogenic and anti-resorptive effects. Currently, the cellular and molecular mechanism underlying the effect of WBV on bone is unclear. In this study, we investigated the response of osteocytes, the putative mechanosensor in bone, under LMHF vibration. As bone cells differentiate from mesenchymal stromal cells (MSCs), we also studied the osteogenic differentiation of rat MSCs in the presence of vibration loading. We found that vibrated osteocytes show gene and protein expression changes suggestive of an anti-osteoclastogenic response, and secrete soluble factors that inhibit osteoclast formation and activity. In contrast, rat MSCs showed moderate to no response to LMHF vibration during osteogenic differentiation. Our data suggest that in vivo effects of LMHF vibration are mediated through mechanosensing and biochemical responses by osteocytes.
URI: http://hdl.handle.net/1807/24596
Appears in Collections:Master
Institute of Biomaterials and Biomedical Engineering - Master theses

Files in This Item:

File Description SizeFormat
Lau_Esther_YT_201006_MASc_thesis.pdf2.18 MBAdobe PDF
View/Open

Items in T-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

uoft