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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/24611

Title: Isolation of ABA Insensitive Mutants using a Sensitized Screen
Authors: Nam, Eric Hyung-Uk
Advisor: McCourt, Peter
Department: Cell and Systems Biology
Keywords: Arabidopsis
Abscisic Acid
ABA
Glucose
Retrograde
Hypocotyl
Light
Signaling
hy
gun
Post-Germination Growth
Issue Date: 27-Jul-2010
Abstract: ABA insensitive mutants (abi1 - abi5) have been isolated in germination screens that use high concentrations of exogenous ABA, and this method is believed to be saturated. To overcome this problem, a sensitized screen that used much lower concentration of exogenous ABA was performed to isolate new ABA insensitive mutants. Some of the isolated mutants had defects in light or retrograde signalling. One particular mutant (18-11) developed long hypocotyls under normal light condition. Based on its response to blue, red and far-red light conditions, this mutant is likely a novel hy mutant. Genetic analysis revealed that while ABA insensitivity in this mutant is recessive, the long hypocotyl phenotype is dominant. Positional cloning is currently being carried out to identify the gene. Findings from this study supports that ABA signalling interacts with light signalling networks.
URI: http://hdl.handle.net/1807/24611
Appears in Collections:Master
Department of Cell and Systems Biology - Master theses

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