test Browse by Author Names Browse by Titles of Works Browse by Subjects of Works Browse by Issue Dates of Works
       

Advanced Search
Home   
 
Browse   
Communities
& Collections
  
Issue Date   
Author   
Title   
Subject   
 
Sign on to:   
Receive email
updates
  
My Account
authorized users
  
Edit Profile   
 
Help   
About T-Space   

T-Space at The University of Toronto Libraries >
School of Graduate Studies - Theses >
Master >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/24626

Title: Vascular Endothelial Growth Factor Functionalized Agarose Can Efficiently Guide Pluripotent Stem Cell Aggregates Toward Blood Progenitor Cells
Authors: Rahman, Muhammad Nafeesur
Advisor: Shoichet, Molly
Zandstra, Peter W.
Department: Chemical Engineering and Applied Chemistry
Keywords: Agarose
Hydrogel
Embryoid Bodies
VEGFA
Blood Progenitor Cells
3D
Differentiation
Serum-Free
Issue Date: 27-Jul-2010
Abstract: Embryonic stem cells (ESCs) are derived from the inner cell mass (ICM) of the embryo that have great potential for regenerative therapies because of their ability to self-renew and differentiate into almost all cell types. However, this developmental potential is influenced by the local cellular microenvironment, including cell surface bound ligands. In this study, we synthesized an artificial stem cell niche wherein vascular endothelial growth factor A (VEGFA) was functionally immobilized in an agarose hydrogel. Immobilized VEGFA treatments were able to upregulate mesodermal markers, brachyury and VEGF receptor 2, by day 4 and were CD34+CD41+ by day seven. Subsequently, VEGFA immobilized treatments were able to generate colony forming cells by day fourteen. This work demonstrates our ability to use functionalized hydrogels to guide ESCs toward blood progenitor cells and serves as a useful tool to replicate aspects of the embryonic microenvironment.
URI: http://hdl.handle.net/1807/24626
Appears in Collections:Master
Department of Chemical Engineering and Applied Chemistry - Master theses

Files in This Item:

File Description SizeFormat
Rahman_Muhammad_Nafeesur_20106_MASc_thesis.pdf5.23 MBAdobe PDF
View/Open

Items in T-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

uoft