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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/24631

Title: Characterization of the E3 Ubiquitin ligase EEL-1 in DNA Damage-induced Germ Line Apoptosis in C. elegans
Authors: Ross, Ashley Jane
Advisor: Derry, Brent
Department: Molecular and Medical Genetics
Keywords: C. elegans
apoptosis
E3 ubiquitin ligases
Issue Date: 28-Jul-2010
Abstract: E3 ubiquitin ligases are important regulators of several cellular processes, including apoptosis. To determine the extent to which E3 ligases regulate DNA damage-induced apoptotic signalling in C. elegans, a high-throughput RNAi screen was performed in our laboratory. We identified the E3 ubiquitin ligase EEL-1 as a positive regulator of DNA damage-induced germ cell apoptosis. ARF-BP1, the mammalian EEL-1 ortholog, negatively regulates both the tumour suppressor protein p53 and the anti-apoptotic protein Mcl-1. In C. elegans, we found that eel-1 regulates DNA damage-induced germ cell apoptosis by a mechanism downstream of cep-1/p53 and upstream of ced-9/mcl-1. My results show that unlike ARF-BP1, EEL-1 does not regulate CED-9/Mcl-1 protein levels, suggesting a novel mechanism of apoptosis regulation in C. elegans for this E3 ligase. Unexpectedly, eel-1 causes synthetic sterility in ced-9 loss-of-function mutants that is suppressed by ablation of the Apaf-1 orthologue ced-4, suggesting an additional role for these genes in oogenesis.
URI: http://hdl.handle.net/1807/24631
Appears in Collections:Master
Department of Molecular Genetics - Master theses

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