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Till, James E. >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/25133

Title: Radiation Sensitivity of Normal and Polyoma-Transformed Rat Embryo Cells
Authors: Williams, J. F.
Till, J. E.
Keywords: embryo cells
polyoma transformed
ionizing radiation
Issue Date: Oct-1966
Publisher: Radiation Research Society
Citation: WILLIAMS JF, TILL JE. Radiation sensitivity of normal and polyoma-transformed rat embryo cells. Radiat Res. 1966;29(2):282-94.
Series/Report no.: Radiation Research
Volume 29 Issue 2
Abstract: Many determinations have been made of the radiosensitivity of the proliferative capacity of mammalian cells. These have been carried out on several different cell lines, both normal and malignant, tested by a variety of techniques, in a number of different laboratories. The results of these determinations (for a summary of data, see ref. 1) have led to the view that normal and malignant mammalian cells derived from different sources differ relatively little in their responses to ionizing radiations, and that the radiosensitivity of cells tested in vitro is similar to that of cells tested in vivo (2-5). Although the available evidence provides strong support for these views, they have not been tested directly with the same cell system, mainly because of the lack of a system suitable for such a test. The finding (6, 7) that rodent embryo cells infected in vitro with polyoma virus may rapidly undergo a transformation in cell properties, and that these "transformed" cells are usually also malignant, in that they are capable of forming tumors when transplanted into suitable adult animals, provided an approach to this problem. Although transformed cells may be obtained from polyoma-infected cultures of either mouse, hamster, or rat embryo cells, the rat embryo cell system appears to be most suitable for radiobiological studies, since quantitative techniques are available for measuring colony formation by both normal and transformed cells in vitro (8), and by malignant cells in vivo (9, 10). Using this system, we have tested for the effect of polyoma virus infection leading to transformation on the radiosensitivity of the colony forming ability of rat embryo cells. Also, the radiosensitivity of cells, based on the survival of their ability to form colonies in vitro, has been compared with that of the same type of cells tested in vivo. The results of these studies are described in this report.
Description: Reproduced with permission from the Radiation Research Society. Radiation Research Society allows published articles to be archived in institutional repositories.
URI: http://www.rrjournal.org/doi/pdf/10.2307/3572012
ISSN: 0033-7587
Appears in Collections:Till, James E.

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