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 Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/25134

 Title: The Effects of X-rays on the Progress of L-Cells through the Cell Cycle Authors: Mak, S.Till, J. E. Keywords: L-cellsDNA synthesisIonizing radiationIrradiation Issue Date: Dec-1963 Publisher: Radiation Research Society Citation: MAK S, TILL JE. Effects of X-rays on progress of L-cells through cell cycle. Radiat Res. 1963;20(4):600-18. Series/Report no.: Radiation ResearchVolume 20 Issue 4 Abstract: It now appears well established that the key components of the genetic mechanism of cells are the macromolecular nucleic acids, and, in particular, macromolecular deoxyribonucleic acid (DNA). It is of considerable interest from a radiobiological point of view to study the effects of ionizing radiation on the functional capabilities of DNA, particularly that of self-replication, which must occur before every mitotic division of a cell. It has been suggested (1, 2) that the principal effect of radiation on the cell cycle of L-cells is on the progress of cells through the post-DNA synthesis (G2) period, though an effect of radiation on the progress of cells through the DNA synthesis period was also observed (2). Since it has been shown in a number of mammalian cell systems that DNA synthesis may be suppressed immediately after irradiation (see, for example, ref. 3-7), it was of interest to study in more detail the effects of radiation on the DNA synthesis period of L-cells. The time relationship between DNA synthesis and mitosis for exponentially multiplying strain-L mouse cells in suspension culture has been determined (8). The approximate durations of the periods of the cell cycle are as follows: mitosis (M) is approximately 1 hour; G1 is 9.0 ± 3.3 hours; the DNA synthesis period (S) is 7.0 ± 1.3 hours; and G2 is 3.0 ± 0.7 hours. (The notation is that of Howard and Pelc, 9.) In this investigation, the effects of X-rays on the progress of strain-L mouse cells in tissue culture from G1 to S, through S and from S to G2 in the first-division cycle after irradiation were studied at the cellular level. The dose of X-rays ranged from 580 to 10,900 rads. The data to be presented indicate that X-radiation has a variable effect on the progress of L-cells through each of the various parts of the division cycle. Description: Reproduced with permission from the Radiation Research Society. Radiation Research Society allows published articles to be archived in institutional repositories. URI: http://www.rrjournal.org/doi/pdf/10.2307/3571352http://dx.doi.org/10.2307/3571352http://hdl.handle.net/1807/25134 ISSN: 0033-7587 Appears in Collections: Till, James E.

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