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Till, James E. >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/25146

Title: Effects of Ionizing Radiation on Cross-Reactivation of T1 Bacteriophage
Authors: Till, J. E.
Pollard, E. C.
Keywords: T1 Bacteriophage
Ionizing radiation
Radiation damage
Genome
Recombinants
Issue Date: Apr-1958
Publisher: Radiation Research Society
Citation: TILL JE, POLLARD EC. Effects of ionizing radiation on cross-reactivation of ti-bacteriophage. Radiat Res. 1958;8(4):344-60.
Series/Report no.: Radiation Research
Volume 8 Issue 4
Abstract: Genetically marked bacteriophage inactivated by radiation are still able to contribute markers to the progeny of mixed infection with active phage of a differing genotype. This phenomenon is termed 'cross-reactivation' and has been studied by several workers, notably Watson (1), Luria (2), Stent (3), Doermann et al. (4), Jacob and Wollman (5), and Stahl (6). Their findings indicate that radiation damage is localized on the genetic material of the phage and leaves part of the genome still functional. Stahl's results, obtained with phage T4 inactivated by the decay of incorporated P32, indicate that p32 decay can damage a considerable portion of the genome. The experiments to be reported below represent an attempt to measure the size and shape of the functional part of the genome remaining after exposure of the phage to ionizing radiation. Under certain specific conditions, the kinetics of the loss of function of biological materials, when exposed to varying doses of different types of ionizing radiation, give a good estimate of the size and shape of the physical structure associated with the particular function. The method has been reviewed by Pollard et al. (7). T1 phage was used in these experiments for the following reasons: (1) It is able to withstand drying without being inactivated. This makes it suitable for radiation studies. (2) It is amenable to genetic analysis (Bresch, 8; Bresch and Mennigmann, 9). (3) The genetic information is apparently carried exclusively on its DNA (Christensen and Tolmach, 10). (4) Some of its properties have already been investigated with the radiation technique (e.g., Fluke and Pollard, 11; Pollard and Setlow, 12). When these experiments were begun, it was not known that cross-reactivation of Tl was possible, and the first objective of the work was to demonstrate that the process could occur. A preliminary account of this demonstration has been published (13). The second objective was to utilize cross-reactivation as a means of detecting the functional portions of the genome.
Description: Reproduced with permission from the Radiation Research Society. Radiation Research Society allows published articles to be archived in institutional repositories.
URI: http://hdl.handle.net/1807/25146
http://www.rrjournal.org/doi/pdf/10.2307/3570473
ISSN: 0033-7587
Appears in Collections:Till, James E.

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