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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/25419

Title: Insulin-induced Suppression of A-type GABA Receptor Signaling in the INS-1 Pancreatic β-cell Line
Authors: Bansal, Pritpal
Advisor: Wang, Qinghua
Lu, Wei-Yang
Department: Physiology
Keywords: GABA
Insulin
Beta-cell
Autocrine
GABA receptor
Islet
Electrophysiology
Issue Date: 14-Dec-2010
Abstract: GABA and GABA type A receptor (GABAAR) are expressed in pancreatic β-cells and comprise an autocrine signaling system. How the GABA-GABAAR system is regulated is unknown. In this study, I investigated insulin’s effect on this system in the INS-1 β-cell line. I found that GABA evoked current (IGABA) in INS-1 cells, resulting in membrane depolarization. Perforated-patch recordings showed that pre-treatment of insulin or zinc-free insulin suppressed IGABA in INS-1 cells (p < 0.01). Radioimmunossay showed that GABA (30 μM) increased C-peptide secretion from INS-1 cells, which was blocked by GABAAR antagonist picrotoxin, indicating that GABA increased insulin secretion through activation of GABAAR. However, insulin significantly reduced the stimulatory effect of GABA on C-peptide secretion (p < 0.05). These data suggest that GABA released from β-cells positively regulates insulin secretion via GABAAR activation, and that insulin negatively regulates the β-cell secretory pathway likely via inhibiting the GABA-GABAAR system in β-cells.
URI: http://hdl.handle.net/1807/25419
Appears in Collections:Master
Department of Physiology - Master theses

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