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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/25426

Title: Characterization of a Novel Interaction Between Septins and the Adenomatous Polyposis Coli Tumor Suppressor.
Authors: Bejide, Margaret Temitope
Advisor: Trimble, William S.
Department: Biochemistry
Keywords: septins
APC
knockdown
abscission
Issue Date: 14-Dec-2010
Abstract: Septins are evolutionarily conserved proteins with roles in chromosome congression and segregation, cytokinesis and microtubule destabilization. Septins form homo- and hetero-oligomeric complexes, which are thought to act as dynamic scaffolds. We identified SEPT2/9/11/10 as novel interacting partners of adenomatous polyposis coli (APC), a bona fide tumor suppressor. Since septins and APC have similar roles and knockdown phenotypes, I sought to determine if they work together to perform their cellular functions. I showed that APC co-immunoprecipitates with endogenous septins in colon cancer cell lines. Using siRNA, I found that SEPT2 and APC may function within the same pathway to regulate DNA congression and segregation. Co-depleting SEPT9 with APC slightly alleviates the chromosome congression and segregation defects caused by siAPC alone. siSEPT9 increased abscission times, which was rescued by co-depleting APC. Future studies should elucidate the significance of the rescue data obtained upon APC and SEPT9 co-depletion and APC’s interactions with SEPT10/11.
URI: http://hdl.handle.net/1807/25426
Appears in Collections:Master
Department of Biochemistry - Master theses

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