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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/25461

Title: Developmental Profile of Hepatic CYP2B Expression, In Vivo and In Vitro Nicotine Metabolism in Rats
Authors: Cui, Zhe
Advisor: Tyndale, Rachel
Department: Pharmacology
Keywords: nicotine
metabolism
rat
CYP2B
developmental
age
adolescent
adult
in vivo
in vitro
Issue Date: 17-Dec-2010
Abstract: Initiation of smoking occurs during adolescence. Some studies found that adolescent rats develop self-administration at higher nicotine doses than adults. For the same nicotine dose, adolescents have lower plasma nicotine levels than adults, suggesting they may have faster nicotine metabolism which may contribute to the differences in nicotine-induced behaviours. This study investigated the developmental differences in rat hepatic CYP2B protein expression, in vivo and in vitro nicotine metabolism. Plasma and brain nicotine levels were lower in early-adolescents than adults, however, cotinine levels were also lower. Slower rates of cotinine formation in early-adolescents may explain their lower cotinine levels. Early-adolescents express higher total CYP2B proteins than adults, but they appear to have less of CYP2B1, the nicotine metabolizing enzyme. These findings provide a better understanding of this animal model and indicate that differences in pharmacokinetics should be considered when comparing nicotine’s effects in adolescents and adults extrapolating behavioural findings to human.
URI: http://hdl.handle.net/1807/25461
Appears in Collections:Master
Department of Pharmacology and Toxicology - Master theses

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