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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/25555

Title: The Role of CtBP in Pituitary Tumorigenesis
Authors: Dorman, Kathryn
Advisor: Asa, Sylvia
Ezzat, Shereen
Department: Laboratory Medicine and Pathobiology
Keywords: Pituitary
CtBP
Chromatin Remodeling
Ikaros
Issue Date: 31-Dec-2010
Abstract: C-terminal Binding Protein (CtBP) is a transcriptional co-repressor that plays an important role in mammalian development and tumorigenesis. CtBP is known to interact with Ikaros, an important transcriptional regulator in the pituitary; however CtBP itself has not been examined in this gland. I examined the role of CtBP in pituitary cell growth and survival. Compared to control pituitary GH4 cells, CtBP1-deficient cells exhibit reduced proliferation and de-regulation of genes involved in cell cycle and growth factor signaling. CtBP1-deficient cells were more susceptible to hypoxia-induced apoptosis and showed a reduction in hypoxia-induced Ikaros expression. Interactions between CtBP and Ikaros isoforms were demonstrated in pituitary tumor cell lines. CtBP and Ikaros also bound a common region of the previously characterized Ikaros target, the LDL-R promoter. These results identify oncogenic properties of CtBP1 in the pituitary and set the groundwork for future studies into regulatory roles of CtBP and Ikaros in the pituitary.
URI: http://hdl.handle.net/1807/25555
Appears in Collections:Master
Department of Laboratory Medicine and Pathobiology - Master theses

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