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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/25556

Title: Innovative Approaches for the Electrochemical Detection of Acetylcholinesterase Inhibitors
Authors: Dounin, Vladimir
Advisor: Kerman, Kagan
Department: Chemistry
Keywords: acetylcholinesterase
electrochemistry
Issue Date: 31-Dec-2010
Abstract: This document describes research conducted during 2009-2010 in the Kerman Group laboratory at the University of Toronto Scarborough to investigate the application of electrochemical techniques for the detection of acetylcholinesterase inhibitors in aqueous samples. Two main projects were completed and are discussed herein. The first project demonstrated that the new unmodified, nanostructured gold disposable electrochemical printed (DEP) chips produced by BioDevice Technology can compete with surface-modified electrode configurations to detect trace concentrations of insecticides. This was achieved through the measurement of acetylcholinesterase-catalyzed production of thiocholine after incubation of the enzyme with low concentrations of paraoxon (10 ppb) and carbofuran (8 ppb). The second project featured the novel application of a glassy carbon (GC) electrode to monitor the changes in availability of Thioflavin T (ThT) for oxidation at the electrode surface, which is non-linearly modulated by the presence of acetylcholinesterase and the enzyme’s pre-treatment with trace concentrations of paraoxon and carbachol.
URI: http://hdl.handle.net/1807/25556
Appears in Collections:Master
Department of Chemistry - Master theses

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