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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/25578

Title: Peripherin-28 as a Biomarker of ALS: A Methodological Study
Authors: Findlater, Joseph
Advisor: Robertson, Janice
Department: Laboratory Medicine and Pathobiology
Keywords: Peripherin
Neurofilament Light
TDP-34
ELISA
amyotrophic lateral sclerosis
Per28
TDP-43 C-Splice
NFL-60
cerebrospinal fluid
biomarkers
alternative splicing
Issue Date: 31-Dec-2010
Abstract: Amyotrophic lateral sclerosis (ALS) is a progressive and fatal neurodegenerative disease which currently lacks rapid and definitive diagnostic tests. Recently identified neuron specific splice variant molecules, Per28 and NFL-60, have been shown to contain unique epitopes and to have altered levels of expression in ALS patients. It is believed that these factors make Per28 and NFL-60 excellent candidate biomarkers for the ALS disease state. In this study, we attempted to develop ELISA assays directed against Per28 and NFL-60, as well as a generalized guideline for splice variant ELISA development, which could be used in a clinical setting. Limitations in currently identified antibodies to the splice variants allowed only for the completion of a Per28 ELISA, which lacked the sensitivity for clinical relevance. This assay creation process, however, did produce a guideline for similar ELISA development, which should allow for the more expeditious creation future ELISA.
URI: http://hdl.handle.net/1807/25578
Appears in Collections:Master
Department of Laboratory Medicine and Pathobiology - Master theses

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