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|Title: ||Microsphere Kinetics in Chronic Total Occlusions|
|Authors: ||Fraser, Ashley|
|Advisor: ||Strauss, Bradley|
|Department: ||Biomedical Engineering|
|Keywords: ||Chronic Total Occlusion|
|Issue Date: ||31-Dec-2010|
|Abstract: ||Chronic total occlusions are a common problem in patients with coronary artery disease. The primary barrier to successful percutaneous coronary intervention is inability to cross the lesion with a guidewire. We seek to characterize polymer microspheres as a controlled delivery mechanism for collagenase and VEGF, novel intralesional therapies being investigated to alter CTO structural properties.
Release profiles for protein-loaded PLGA [poly(lactic-co-glycolic acid)] microspheres showed sustained BSA and VEGF release over eight and 48 hours respectively. Polymer degradation products had no impact on endothelial cell growth and protein bioactivity was maintained post-release. In vivo localization of microsphere-released collagenase was not possible due to low concentrations remaining at the site. Histology confirmed microspheres remained in the collagen-dense, proximal 15 mm of the lesion, likely altering the structural integrity of the plaque.|
|Appears in Collections:||Master|
Institute of Biomaterials and Biomedical Engineering - Master theses
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