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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/25605

Title: Reduced Burst Release of Bioactive rhBMP-2 from a Three-phase Composite Scaffold
Authors: Grant, David William
Advisor: Davies, John Edward
Department: Biomedical Engineering
Keywords: Drug delivery
Bone
Bone morphogenic protein
BMP
BMP-2
rhBMP-2
bone graft
tissue engineering
scaffold
bone tissue engineering
regenerative medicine
osteoinduction
osteoconduction
osteogenesis
generative surgery
autoraft replacement
synthetic autograft replacement
morphogenic bioactivity assay
protein release kinetics
bovine serum albumin
model protein
biomaterials
biomaterial surface analysis
scanning electron microscope application
x-ray photoelectron spectroscopy application
accelerating voltage application
burst release
sustained kinetics
sustained delivery
composite scaffold
three phase scaffold
PLGA
calcium phosphate
mineral-polymer composite
sterilization
BMP sterilization
long-term storage
BMP storage
Issue Date: 31-Dec-2010
Abstract: Recombinant human bone morphogenic proteins (rhBMPs) are extensively studied and employed clinically for treatment of various bone defects. Current clinical delivery vehicles suffer wasteful burst releases that mandate supra-physiological dosing driving concerns over safety and cost. It was therefore investigated whether a unique drug delivery vehicle sequestered within a composite scaffold could lower the burst release of rhBMP-2. PLGA-calcium phosphate tri-phasic composite scaffolds delivered model protein BSA with burst release of ~13% and sustained kinetics of 0.5-1.5% BSA/day up to 45 days. rhBMP-2 was delivered with zero burst release however at much lower levels, totaling 0.09% to 0.9 % release over 10 days, but had up to 6.3-fold greater bioactivity than fresh rhBMP-2 (p<0.05). In conclusion, the three-phase composite scaffold can deliver bioactive proteins with a reduced burst release and sustained secondary kinetics.
URI: http://hdl.handle.net/1807/25605
Appears in Collections:Master
Institute of Biomaterials and Biomedical Engineering - Master theses

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