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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/25607

Title: Redistribution of PKC{epsilon} to the Mitochondria: Comparing Myocardial Ischemic and Pharmacologic Preconditioning
Authors: Habbous, Steven
Advisor: Wilson, Gregory J.
Department: Medical Science
Keywords: Preconditioning
Ischemia
Langendorff
PKC
Mitochondria
epsilon
GPCR
Immunogold
Translocation
Redistribution
Reperfusion
Signaling
Adenosine
Bradykinin
Opioid
Angiotensin II
Issue Date: 31-Dec-2010
Abstract: PKCe plays a very important role in mediating the protection against myocardial ischemia and reperfusion injury induced by ischemic preconditioning (IPC) and pharmacologic preconditioning (PPC). The redistribution of PKCe was assessed by subcellular fractionation and western blotting in the Langendorff-perfused rabbit heart. Either 5min ischemia or 5min administration of adenosine A1 and/or A3 agonists, bradykinin, angiotensin II, and d1-opioid agonists resulted in PKCe redistribution from the cytosol to the mitochondria. This effect of IPC on PKCe redistribution was visible up to at least 30min of reperfusion, while that of PPC was lost by 10min of drug washout, indicative of the transient nature of PKCe redistribution. PKCe redistribution to mitochondria by IPC was also visualized using immunogold electron microscopy. Thus, IPC and PPC caused PKCe redistribution from the cytosol to the mitochondria, which was longer-lasting in IPC than in PPC.
URI: http://hdl.handle.net/1807/25607
Appears in Collections:Master
Institute of Medical Science - Master theses

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