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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/25614

Title: Adseverin is a Key Regulator and Marker of Osteoclastogenesis
Authors: Hassanpour, Siavash
Advisor: Glogauer, Michael
Department: Dentistry
Keywords: Osteoclast
Issue Date: 1-Jan-2011
Abstract: The intricate processes of osteoclastogenesis are highly dependent on the dynamic regulation of the actin cytoskeleton. Adseverin, a member of the gelsolin superfamily of actin binding proteins, regulates actin remodeling by severing and capping actin filaments in a calcium dependent manner. The objective of this project was to characterize the role(s) of adseverin during osteoclastogenesis, by assessing adseverin expression throughout osteoclastogenesis and through differentiation assays using a knockdown strategy. Methods: qRT-PCR and immunoblot analyses were used to examine adseverin expression during osteoclastogenesis. A stable adseverin knockdown macrophage cell line was generated using a retroviral shRNA construct. Results: Adseverin expression increased significantly in response to RANKL during the early phases of osteoclastogenesis, and adseverin was highly expressed in mature osteoclasts. Adseverin knockdown macrophages experienced a major osteoclastogenesis defect, most likely caused by a defect in pre-osteoclast fusion. Conclusion: Adseverin is a RANKL induced early and pro-fusion marker of osteoclastogenesis.
URI: http://hdl.handle.net/1807/25614
Appears in Collections:Master
Faculty of Dentistry - Master theses

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