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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/25622

Title: Characterization of the Interactome of BTB Domains
Authors: Hu, Yaqi
Advisor: PrivĂ©, Gilbert G.
Department: Biochemistry
Keywords: Proteomics
Protein Interaction
Issue Date: 1-Jan-2011
Abstract: The BTB domain is a well-conserved protein-protein interaction motif. There are 43 BTB-ZF transcription factors in the human proteome. Many of these transcription factors play crucial roles in cancer and developmental processes. The purpose of this project is to identify lists of interactors of the BTB domains of six BTB-ZF proteins with high confidence using a mass spectrometry based approach. The BTB domains BCL6, PLZF, Kaiso, LRF, FAZF, and Miz1 were studied. This study was able to identify 142 putative interactors. The list of putative interactor proteins participates in a wide array of biological functions. Selected putative interactors of the BCL6BTB were also validated using biochemical techniques. In conclusion, this project was able to provide an analysis of the protein-protein interactions mediated by the BTB domains of six BTB-ZF transcription factors. The information generated is valuable to guide future functional and structural studies of the BTB domains.
URI: http://hdl.handle.net/1807/25622
Appears in Collections:Master
Department of Biochemistry - Master theses

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