test Browse by Author Names Browse by Titles of Works Browse by Subjects of Works Browse by Issue Dates of Works

Advanced Search
& Collections
Issue Date   
Sign on to:   
Receive email
My Account
authorized users
Edit Profile   
About T-Space   

T-Space at The University of Toronto Libraries >
School of Graduate Studies - Theses >
Master >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/25629

Title: Domain Boundaries are Essential for the Solubility of Nucleotide Binding Domains of ABC Transporters
Authors: Ikeda, Lynn Kumiko
Advisor: Kanelis, Voula
Department: Chemistry
Keywords: Biochemistry
ABC Transporters
Nucleotide Binding Domain
Sulfonylurea Receptors
KATP channels
Issue Date: 1-Jan-2011
Abstract: SUR2A is a member of the ABC transporter superfamily. SUR2A mediated regulation of KATP channels is essential as mutations in the nucleotide binding domains (NBDs) of SUR2A are associated with cardiovascular disorders. Studies of eukaryotic NBDs, such as SUR2A, are hindered by low solubility of the isolated domain. We hypothesized that the solubility of heterologously expressed SUR2A NBDs depends on the definition of the domain boundaries. Boundaries were initially predicted using a combination of a structure-based sequence alignment and homology modeling, and subsequently verified by testing the solubility of five SUR2A NBD1 constructs with different N- or C-terminal boundaries. The boundaries of SUR2A NBD1 essential for solubility were identified. CD and NMR data indicate that SUR2A NBD1 is folded. Our method may be applied as a general method for developing suitable constructs of other NBDs of ABC proteins such as SUR isoforms, SUR2B and SUR2C, and the vacuolar transporter, Ycf1p.
URI: http://hdl.handle.net/1807/25629
Appears in Collections:Master
Department of Chemistry - Master theses

Files in This Item:

File Description SizeFormat
Ikeda_Lynn_K_201011_MSc_thesis.pdf3.57 MBAdobe PDF

Items in T-Space are protected by copyright, with all rights reserved, unless otherwise indicated.