test Browse by Author Names Browse by Titles of Works Browse by Subjects of Works Browse by Issue Dates of Works

Advanced Search
& Collections
Issue Date   
Sign on to:   
Receive email
My Account
authorized users
Edit Profile   
About T-Space   

T-Space at The University of Toronto Libraries >
School of Graduate Studies - Theses >
Master >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/25633

Title: Epigenetic Basis for Heterogeneity in VCAM-1 Gene Expression Patterns in Cytokine Activated Vascular Endothelium
Authors: Jamal, Alisha Noorin
Advisor: Marsden, Philip A.
Department: Laboratory Medicine and Pathobiology
Keywords: Pathology
Vascular Endothelium
Issue Date: 1-Jan-2011
Abstract: Vascular cell adhesion molecule-1 (VCAM-1) is a cytokine-activated protein present on endothelial cells (ECs). Our laboratory has provided evidence that DNA methylation, a mark associated with gene silencing, is fundamental for regulating VCAM-1 expression. First, we showed that RNA polymerase II, preferentially associates with VCAM-1 hypomethylated alleles. This finding was confirmed using fluorescence-activated cell sorting (FACS) to sort populations of cytokine-activated ECs with high vs. low cell surface VCAM-1 expression. We found that ECs with high VCAM-1 expression were hypomethylated at the promoter. We then went on to show that populations of cells generated from single ECs exhibit differential VCAM-1 methylation from one another, and from the original founder population. Intriguingly, our data shows that VCAM-1 mRNA levels differ between the clones, and correlate with the observed differences in DNA methylation. Taken together, this data provides exciting evidence that DNA methylation is important in the regulation of VCAM-1 gene expression.
URI: http://hdl.handle.net/1807/25633
Appears in Collections:Master
Department of Laboratory Medicine and Pathobiology - Master theses

Files in This Item:

File Description SizeFormat
Jamal_Alisha_N_201011_MSc_thesis.pdf11.43 MBAdobe PDF

This item is licensed under a Creative Commons License
Creative Commons

Items in T-Space are protected by copyright, with all rights reserved, unless otherwise indicated.