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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/25700

Title: A Method to Identify DNA Sequences Contained in an Arbitrary Nuclear Region
Authors: Anchel, David
Advisor: Bazett-Jones, David
Department: Biochemistry
Keywords: general biology
genetics
molecular biology
Issue Date: 3-Jan-2011
Abstract: Nuclear substructures known as “nuclear bodies” associate with particular gene loci, and this may determine or reflect a mechanism of genetic control. The detection of these associations currently relies on the use of loci-specific probes, or immunoprecipitation of bulk cells with a particular protein. However, there is evidence that these associations respect the nuclear body, not necessarily any one constituent protein, and are statistically and/or functionally significant even when they occur over distances resolvable by light microscopy, or within single cells. A method is proposed that will allow for the identification of loci contained within the vicinity of an arbitrary nuclear structure in a single cell. It is demonstrated that the crucial aspects of this method are feasible; that DNA sequences originating from arbitrary subnuclear regions targeted by two-photon irradiation can be determined, and identified to a particular locus.
URI: http://hdl.handle.net/1807/25700
Appears in Collections:Master
Department of Biochemistry - Master theses

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