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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/25727

Title: The Hormonal Regulation of Kisspeptin and Neuropeptide Y Hypothalamic Neurons
Authors: Kim, Ginah
Advisor: Belsham, Denise
Department: Physiology
Keywords: kisspeptin
neuropeptide Y
estrogen
hypothalamus
immortalized cell lines
Issue Date: 6-Jan-2011
Abstract: Kisspeptin (encoded by Kiss1) is a hypothalamic neuropeptide that is directly regulated by sex steroids and directly stimulates gonadotropin-releasing hormone (GnRH) neurons. Kisspeptin cell models were established in order to facilitate future molecular analysis of kisspeptin. mHypoA-51 and mHypoA-63 cell lines were found to express kisspeptin, estrogen receptor α and β, substance P, but not tyrosine hydroxyase. Furthermore, estrogen decreased Kiss1 expression in both cell lines. Based on these results, it was concluded that mHypoA-51 and mHypoA-63 are representative of arcuate kisspeptin neurons. Accumulating evidence also indicates that kisspeptin indirectly stimulates GnRH neurons through afferent neurons. Kisspeptin receptor expression was detected in native neuropeptide Y (NPY) neurons. Using the mHypoE-38 cell line, kisspeptin was found to directly regulate NPY mRNA expression and secretion via the ERK1/2 and p38 MAPK pathways. This is the first evidence that kisspeptin directly stimulates NPY neurons to potentially exert indirect effects on GnRH neurons.
URI: http://hdl.handle.net/1807/25727
Appears in Collections:Master
Department of Physiology - Master theses

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