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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/25730

Title: Investigating the Process of Cement Line Maturation on Substrate Surfaces with Submicron Undercuts
Authors: Ko, James Chih-Hsien Jr.
Advisor: Davies, John Edward
Department: Dentistry
Keywords: Cement Line
Bone Implant Interface
Submicron Undercuts
Issue Date: 6-Jan-2011
Abstract: The cement line is the first mineralized matrix deposited on an implant surface during contact osteogenesis forming the bone/implant interface. The hypothesis underlying the present project was that non-collagenous cement line proteins must be deposited into the submicron undercuts on substrate surfaces prior mineralization. In vitro osteogenic cultures were used to grow bone nodules on Thermanox┬« coverslips modified with calcium phosphate nanocrystals, creating an undercutted surface. Electron microscopy was used to observe cement line formation. BSP immunogold labelling was used to determine if the cement line organic matrix is deposited within undercuts prior mineralization. The results showed the deposited bone nodules, and on test coverslips the deposited cement line was thicker and evenly distributed than control. Furthermore, positive BSP labelling was found within the undercuts prior to cement line mineralization. Thus, it can be concluded that cement line proteins are deposited into submicron undercuts on substrate surfaces prior to mineralization.
URI: http://hdl.handle.net/1807/25730
Appears in Collections:Master
Faculty of Dentistry - Master theses

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