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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/25742

Title: A Phage Display System to Profile the DNA-binding Specificities of C2H2 Zinc Fingers
Authors: Lam, Kathy
Advisor: Hughes, Timothy R.
Department: Molecular and Medical Genetics
Keywords: C2H2
zinc fingers
phage display
modular
DNA-binding specificity
Issue Date: 7-Jan-2011
Abstract: Knowing the sequence specificities of transcription factors allows us to surmise their functions and establish their regulatory roles in genomes. The most common DNA-binding domain among eukaryotic transcription factors is the Cys2His2 zinc finger domain; however, despite their prevalence, the specificities of the majority of Cys2His2 zinc finger proteins remain unknown due to the difficulty in assaying them. My objective was to develop a new phage displayed-based assay, in which individual Cys2His2 domains are displayed on phage in an otherwise constant three-finger protein scaffold. In Chapter 2, I discuss evidence for the modularity of the Cys2His2 domain, since my assay requires that zinc fingers be modular. In Chapter 3, I describe my results on the development of this phage display-based assay. This work provides support for a new strategy to determine the specificities of individual zinc fingers, which can be used to infer specificities for multi-finger Cys2His2 proteins.
URI: http://hdl.handle.net/1807/25742
Appears in Collections:Master
Department of Molecular Genetics - Master theses

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