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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/25743

Title: Characterizing the Organization within Alternative Lengthening of Telomere Associated-promyelocytic Leukemia Nuclear Bodies
Authors: Larsen, Andrew
Advisor: Bazett-Jones, David
Department: Biochemistry
Keywords: Alternative Lengthening of Telomeres
Promyelocytic Leukemia Nuclear Bodies
Electron Spectroscopic Imaging
Nuclear Organization
Issue Date: 7-Jan-2011
Abstract: In the absence of telomerase activity, a subset of cancerous and immortalized cells maintain telomere length by means of a poorly understood mechanism, termed alternative lengthening of telomeres (ALT). Many details of telomere maintenance in ALT positive cells remain unclear, but significant evidence implicates a homologous recombination mechanism. ALT specific nuclear structures, known as ALT-associated promyelocytic leukemia nuclear bodies (APBs), are thought to serve as the site of telomere extension. Using electron spectroscopic imaging we have demonstrated that APBs contain substantial amounts of nucleic acid sequestered within the bodies. In contrast, promyelocytic leukemia nuclear bodies in non-ALT cell lines contain no significant nucleic acid. We show that the nucleic acid found in APBs is not RNA and provide evidence that it is in fact telomeric repeat DNA. This evidence supports a role for APBs to sequester extrachromosomal telomeric DNA in order to suppress the activation of DNA repair.
URI: http://hdl.handle.net/1807/25743
Appears in Collections:Master
Department of Biochemistry - Master theses

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