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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/25752

Title: Reversal of Morphine-induced Locomotion in M5 Muscarinic Receptor Knockout Mice with Food Deprivation but not Bilateral Infusions of VTA BDNF
Authors: Lee, Esther
Advisor: Yeomans, John S.
Department: Cell and Systems Biology
Keywords: muscarinic
M5
acetylcholine
dopamine
food deprivation
knockout
mice
morphine
locomotion
brain-derived neurotrophic factor
mesolimbic
pedunculopontine tegmental nucleus
open-field
Issue Date: 7-Jan-2011
Abstract: Cholinergic inputs from mesopontine tegmentum activate midbrain dopamine (DA) neurons via M5 muscarinic receptors. The M5 receptor is important for mesopontine stimulation-induced accumbal or striatal DA efflux, brain stimulation reward or morphine-induced conditioned place preference (CPP). M5 receptor knockout (KO) mice show 40-50% less morphine-induced locomotion. Pedunculopontine tegmental nucleus (PPT) lesions in rodents block morphine CPP, but are ineffective after 18 hours food deprivation, opiate dependence, or intra-VTA BDNF. Based on these findings, we investigated whether acute food deprivation or intra-VTA BDNF alters morphine-induced locomotion (3 and 10 mg/kg, i.p.) in C57BL/6 M5 KO mice. Non-deprived M5 KOs showed reduced morphine-induced locomotion, suggesting M5 receptors partly mediate morphine-induced locomotion. Morphine-induced locomotion was reversed in food-deprived mice, suggesting the stimulant effects of morphine were altered to bypass the PPT. Unexpectedly, intra-VTA BDNF infusions were ineffective in altering morphine-induced locomotion. Additionally, M5 KOs receiving intra-VTA saline showed no deficits in morphine-induced locomotion.
URI: http://hdl.handle.net/1807/25752
Appears in Collections:Master
Department of Cell and Systems Biology - Master theses

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