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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/25756

Title: Genetic Factors Influencing BCG Vaccine Properties
Authors: Leung, Andrea
Advisor: Liu, Jun
Department: Molecular and Medical Genetics
Keywords: BCG
Mycobacterium tuberculosis
Issue Date: 10-Jan-2011
Abstract: Tuberculosis is a re-emerging global health problem. Bacille Calmette-Guerin (BCG), the available vaccine against the disease, is only effective short term and is associated with adverse reactions clinically. The development of new effective vaccines will require an understanding of virulence, immunogenic factors and the beneficial immune responses induced in the human host. My thesis investigates phoP and whiB3, two genes associated with virulence and immunogenicity in Mycobacterium tuberculosis. Study of PhoP in a natural phoP mutant, BCG-Prague, and in the clinically safe BCG-Japan, shows that over-expression of PhoP increases the immunogenicity of these vaccine strains. In addition, I found that WhiB3 impacts carbon metabolism in BCG-Birkhaug and BCG-Sweden, although the effect of this on virulence in vivo is still unclear. The characterization of genes involved in virulence and immunogenicity allows us to develop novel approaches for improving the efficacy of BCG, which has important implications for future TB vaccine development.
URI: http://hdl.handle.net/1807/25756
Appears in Collections:Master
Department of Molecular Genetics - Master theses

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