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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/25768

Title: Characterization of Murine Intestinal Mucus Layer Gene Expression during Postnatal Maturation
Authors: Lin, Aifeng
Advisor: Comelli, Elena
Department: Nutritional Sciences
Keywords: Nutrition
Issue Date: 10-Jan-2011
Abstract: The objective of this work was to characterize the expression pattern of the main intestinal mucin and glycosyltransferases genes in the distal ileum, caecum, proximal and distal colon of 5, 10, 15, 21, 25 and 32 days old mouse pups by real-time PCR. We found that all genes considered, except for Muc13 followed a spatio-temporal expression pattern. As a potential modulator of mucin gene expression, gut microbiota composition was also analyzed in the same animals. We found that neither the total bacterial numbers nor the relative abundance of the main microbial phyla vary after 21 days of age. The characterization of the postnatal intestinal mucus layer gene expression may aid to understand disease-related deviations and help designing nutritional interventions to sustain the intestinal barrier.
URI: http://hdl.handle.net/1807/25768
Appears in Collections:Master
Department of Nutritional Sciences - Master theses

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