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|Title: ||Multiplication of Animal Cells in Suspension Measured by Colony Counts|
|Authors: ||Gwatkin, R. B. L.|
Till, J. E.
Whitmore, G. F.
Graham, A. F.
|Keywords: ||cultivation of animal cells|
|Issue Date: ||Jun-1957|
|Publisher: ||National Academy of Sciences USA|
|Citation: ||GWATKIN RBL, TILL JE, WHITMORE GF, SIMINOVITCH L, GRAHAM AF. Multiplication of animal cells in suspension measured by colony counts. Proc Natl Acad Sci U S A. 1957;43(6):451-7.|
|Series/Report no.: ||Proceedings of the National Academy of Sciences USA|
Vol. 43 No. 6
|Abstract: ||During the past few years the development of new techniques for the cultivation
of animal cells in vitro has facilitated the quantitative study of many aspects of
cell biology. At present the most commonly used method of propagating cell
strains is based on the ability of cells to multiply while attached to a glass surface.
The cells may be subcultured by removing them from the surface into suspension
and then distributing them into other vessels, where they will again adhere to the
glass and populate the surface. This procedure has been developed by Earle and
his associates into the so-called quantitative replicate culture technique and
applied to a variety of studies with animal cells. Despite the technical advance
represented by this method, there are, nevertheless, serious experimental limitations inherent in the use of glass surfaces for cultivating large cell populations. Perhaps
the most obvious of these is the problem of removing representative samples at
will during the growth of a cell population. In addition, subculture requires the
removal of cells from the surface, with consequent risk of cell injury.|
|Description: ||Archived with permission from the National Academy of Sciences USA. Originally published in Proceedings of the National Academy of Sciences USA volume 43 issue 6. Please refer to www.pnas.org for this series of publications. Author holds all copyright for this article.|
|Appears in Collections:||Till, James E.|
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