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Till, James E. >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/25976

Title: Multiplication of Animal Cells in Suspension Measured by Colony Counts
Authors: Gwatkin, R. B. L.
Till, J. E.
Whitmore, G. F.
Siminovitch, L.
Graham, A. F.
Keywords: cultivation of animal cells
colony counts
Issue Date: Jun-1957
Publisher: National Academy of Sciences USA
Citation: GWATKIN RBL, TILL JE, WHITMORE GF, SIMINOVITCH L, GRAHAM AF. Multiplication of animal cells in suspension measured by colony counts. Proc Natl Acad Sci U S A. 1957;43(6):451-7.
Series/Report no.: Proceedings of the National Academy of Sciences USA
Vol. 43 No. 6
Abstract: During the past few years the development of new techniques for the cultivation of animal cells in vitro has facilitated the quantitative study of many aspects of cell biology. At present the most commonly used method of propagating cell strains is based on the ability of cells to multiply while attached to a glass surface. The cells may be subcultured by removing them from the surface into suspension and then distributing them into other vessels, where they will again adhere to the glass and populate the surface. This procedure has been developed by Earle and his associates into the so-called quantitative replicate culture technique and applied to a variety of studies with animal cells. Despite the technical advance represented by this method, there are, nevertheless, serious experimental limitations inherent in the use of glass surfaces for cultivating large cell populations. Perhaps the most obvious of these is the problem of removing representative samples at will during the growth of a cell population. In addition, subculture requires the removal of cells from the surface, with consequent risk of cell injury.
Description: Archived with permission from the National Academy of Sciences USA. Originally published in Proceedings of the National Academy of Sciences USA volume 43 issue 6. Please refer to www.pnas.org for this series of publications. Author holds all copyright for this article.
URI: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC528479/?page=1
http://www.pnas.org
http://hdl.handle.net/1807/25976
ISSN: 0027-8424
Appears in Collections:Till, James E.

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