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|Title: ||Evidence for a Relationship between Mouse Hemopoietic Stem Cells and Cells Forming Colonies in Culture|
|Authors: ||Wu, A. M.|
Till, J. E.
McCulloch, E. A.
|Keywords: ||hemopoietic stem cells|
spleen colony method
|Issue Date: ||15-Apr-1968|
|Publisher: ||National Academy of Sciences|
|Citation: ||WU, A. M., SIMINOVI.L, TILL, J. E., & MCCULLOC.EA. (1968). Evidence for a relationship between mouse hemopoietic stem cells and cells forming colonies in culture. Proceedings of the National Academy of Sciences of the United States of America, 59(4), 1209-1215.|
|Series/Report no.: ||Proceedings of the National Academy of Sciences of USA|
Vol. 59 No. 4
|Abstract: ||The spleen colony technique has proved to be a useful tool for the enumeration
of hemopoietic stem cells in the mouse and for the study of their properties.
However, since the method depends on colony formation in the spleens of
heavily irradiated' or genetically anemic mice, it has certain inherent limitations.
For example, events occurring early in the growth of colonies cannot be observed,
since they are obscured by the splenic environment; further, the spleen colony
method has not been applied successfully to the study of human cells
and, therefore, the information obtained by use of this method cannot be applied
directly to clinical problems. These limitations might be avoided by the
use of cell-culture methods. Recently, Pluznik and Sachs and Bradley and
Metcalf have reported that cells derived from mouse hemopoietic tissue will
form colonies in culture if they are suspended in dilute agar over a firm agar
base containing a source of suitable "conditioning factor." If a relationship
were established between the cells that give rise to these colonies in culture and
the hemopoietic stem cells responsible for colony formation in the spleen, the
two techniques might complement each other. Results obtained by the use of
the culture technique might be related through the spleen-colony method to
normal and abnormal hemopoiesis in vivo, and the information gained in the mouse
by using the spleen-colony technique might be extended to other species, including
man, by using the culture method.|
|Description: ||Originally published in Vol 59 No. 4 of the Proceedings of the National Academy of Sciences of USA. The author(s) retains all copyright for works published in volume 1-89.|
|Appears in Collections:||Till, James E.|
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