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Till, James E. >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/26184

Title: Evidence for a Relationship between Mouse Hemopoietic Stem Cells and Cells Forming Colonies in Culture
Authors: Wu, A. M.
Siminovi, L.
Till, J. E.
McCulloch, E. A.
Keywords: hemopoietic stem cells
spleen colony method
Issue Date: 15-Apr-1968
Publisher: National Academy of Sciences
Citation: WU, A. M., SIMINOVI.L, TILL, J. E., & MCCULLOC.EA. (1968). Evidence for a relationship between mouse hemopoietic stem cells and cells forming colonies in culture. Proceedings of the National Academy of Sciences of the United States of America, 59(4), 1209-1215.
Series/Report no.: Proceedings of the National Academy of Sciences of USA
Vol. 59 No. 4
Abstract: The spleen colony technique has proved to be a useful tool for the enumeration of hemopoietic stem cells in the mouse and for the study of their properties. However, since the method depends on colony formation in the spleens of heavily irradiated' or genetically anemic mice, it has certain inherent limitations. For example, events occurring early in the growth of colonies cannot be observed, since they are obscured by the splenic environment; further, the spleen colony method has not been applied successfully to the study of human cells and, therefore, the information obtained by use of this method cannot be applied directly to clinical problems. These limitations might be avoided by the use of cell-culture methods. Recently, Pluznik and Sachs and Bradley and Metcalf have reported that cells derived from mouse hemopoietic tissue will form colonies in culture if they are suspended in dilute agar over a firm agar base containing a source of suitable "conditioning factor." If a relationship were established between the cells that give rise to these colonies in culture and the hemopoietic stem cells responsible for colony formation in the spleen, the two techniques might complement each other. Results obtained by the use of the culture technique might be related through the spleen-colony method to normal and abnormal hemopoiesis in vivo, and the information gained in the mouse by using the spleen-colony technique might be extended to other species, including man, by using the culture method.
Description: Originally published in Vol 59 No. 4 of the Proceedings of the National Academy of Sciences of USA. The author(s) retains all copyright for works published in volume 1-89.
URI: http://www.pnas.org/
ISSN: 0027-8424
Appears in Collections:Till, James E.

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