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|Title: ||Interacting Cell-Populations in Cultures of Leukocytes from Normal Or Leukemic Peripheral-Blood|
|Authors: ||McCulloch, E. A.|
Till, J. E.
|Issue Date: ||Feb-1977|
|Publisher: ||American Society of Hematology|
|Citation: ||MCCULLOCH, E. A., & TILL, J. E. (1977). Interacting cell-populations in cultures of leukocytes from normal or leukemic peripheral-blood. Blood, 49(2), 269-280.|
|Series/Report no.: ||Blood|
Vol. 49 No.2
|Abstract: ||Kinetic studies in cultures containing 2 X 10(5) peripheral leukocytes from patients with acute myelobastic leukemia revealed extensive, radiation-sensitive increases in thymidine incorporation without parallel increases in cell number. Modest and variable stimulation of 3HTdR incorporation was seen with the addition of either leukocyte- conditioned medium prepared with phytohemagglutin (PHA) or PHA alone. However, using the method of limiting dilution, stimulation was always observed and ranged from 3- to 20-fold in individual patients. By mixing small numbers of intact cells with larger numbers of irradiated autologous cells, quantitative evidence was obtained for a cellular interaction between irradiated, PHA-stimulated populations capable of 3HTdR incorporation. Similar evidence for cell-cell interaction was obtained for normal leukocytes.|
|Description: ||This research was originally published in Blood. MCCULLOCH, E. A., & TILL, J. E. (1977). Interacting cell-populations in cultures of leukocytes from normal or leukemic peripheral-blood. Blood, 49(2), 269-280. © the American Society of Hematology.|
|Appears in Collections:||Till, James E.|
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