test Browse by Author Names Browse by Titles of Works Browse by Subjects of Works Browse by Issue Dates of Works
       

Advanced Search
Home   
 
Browse   
Communities
& Collections
  
Issue Date   
Author   
Title   
Subject   
 
Sign on to:   
Receive email
updates
  
My Account
authorized users
  
Edit Profile   
 
Help   
About T-Space   

T-Space at The University of Toronto Libraries >
University Professors >
Till, James E. >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/26397

Title: Interacting Cell-Populations in Cultures of Leukocytes from Normal Or Leukemic Peripheral-Blood
Authors: McCulloch, E. A.
Till, J. E.
Issue Date: Feb-1977
Publisher: American Society of Hematology
Citation: MCCULLOCH, E. A., & TILL, J. E. (1977). Interacting cell-populations in cultures of leukocytes from normal or leukemic peripheral-blood. Blood, 49(2), 269-280.
Series/Report no.: Blood
Vol. 49 No.2
Abstract: Kinetic studies in cultures containing 2 X 10(5) peripheral leukocytes from patients with acute myelobastic leukemia revealed extensive, radiation-sensitive increases in thymidine incorporation without parallel increases in cell number. Modest and variable stimulation of 3HTdR incorporation was seen with the addition of either leukocyte- conditioned medium prepared with phytohemagglutin (PHA) or PHA alone. However, using the method of limiting dilution, stimulation was always observed and ranged from 3- to 20-fold in individual patients. By mixing small numbers of intact cells with larger numbers of irradiated autologous cells, quantitative evidence was obtained for a cellular interaction between irradiated, PHA-stimulated populations capable of 3HTdR incorporation. Similar evidence for cell-cell interaction was obtained for normal leukocytes.
Description: This research was originally published in Blood. MCCULLOCH, E. A., & TILL, J. E. (1977). Interacting cell-populations in cultures of leukocytes from normal or leukemic peripheral-blood. Blood, 49(2), 269-280. © the American Society of Hematology.
URI: http://hdl.handle.net/1807/26397
ISSN: 0006-4971
http://bloodjournal.hematologylibrary.org/
http://bloodjournal.hematologylibrary.org/cgi/content/abstract/49/2/269
Appears in Collections:Till, James E.

Files in This Item:

File Description SizeFormat
Interacting cell populations in cultures of leukocytes from normal or leukemic peripheral blood.pdf1.48 MBAdobe PDF
View/Open

Items in T-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

uoft