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Till, James E. >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/26407

Title: Effect of Differing Demands for Blood Cell Production on Dna Synthesis by Hemopoietic Colony-Forming Cells of Mice
Authors: Becker, A. J.
McCulloch, E. A
Sminovitch, L.
Till, J. E.
Issue Date: Sep-1965
Publisher: American Society of Hematology
Citation: BECKER, A. J., MCCULLOC.EA, SIMINOVI.L, & TILL, J. E. (1965). Effect of differing demands for blood cell production on dna synthesis by hemopoietic colony-forming cells of mice. Blood-the Journal of Hematology, 26(3), 296-308.
Series/Report no.: Blood
Vol. 26 No. 3
Abstract: A technic capable of estimating the fraction of hemopoietic colony-forming progenitor cells in DNA synthesis in vivo has been described. The technic is based on the ability of tritiated thymidine to inhibit the growth of those colony-forming cells which, by virtue of their presence in the S-phase during a 20-minute incubation in vitro, in the presence of 500 µc./ml. of H3TdR, have incorporated large amounts of the nucleoside. The method has been applied to transplanted colony-forming cells proliferating in spleens of heavily irradiated recipients as well as to cells from normal fetal liver, normal marrow, and normal spleen. In situations where the hemopoietic system is expanding (fetal liver and regenerating transplants), a large fraction, 40-65 per cent, of the stem cells are in DNA synthesis. In the steady-state situations (adult marrow and spleen), the fraction of cells in DNA synthesis is almost imperceptible using this technic. It is concluded that control mechanisms which govern the rate of hemopoiesis operate, at least in part, by altering the generative cycle of blood-forming progenitor cells.
Description: This research was originally published in Blood. BECKER, A. J., MCCULLOC.EA, SIMINOVI.L, & TILL, J. E. (1965). Effect of differing demands for blood cell production on dna synthesis by hemopoietic colony-forming cells of mice. Blood-the Journal of Hematology, 26(3), 296-308. © the American Society of Hematology.
URI: http://bloodjournal.hematologylibrary.org/
http://bloodjournal.hematologylibrary.org/cgi/content/abstract/26/3/296
http://hdl.handle.net/1807/26407
ISSN: 0006-4971
Appears in Collections:Till, James E.

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