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T-Space at The University of Toronto Libraries >
School of Graduate Studies - Theses >
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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/27463

Title: The Response of Annulus Fibrosus Cells to Fibronectin- Coated Nanofibrous Polyurethrane-Carbonate Anionic Dihydroxyoligomer Scaffolds
Authors: Attia, Menat
Advisor: Kandel, Rita A.
Santerre, J. Paul
Department: Laboratory Medicine and Pathobiology
Keywords: annulus fibrosus
tissue engineering
nanofibrous scaffolds
polyurethane
Issue Date: 1-Jun-2011
Abstract: Tissue engineering of the annulus fibrosus (AF) is challenging due to its complex lamellar structure. Polyurethane scaffolds have shown promise in AF tissue engineering. The current study examines whether matrix protein coatings (collagen type I, fibronectin, or vitronectin) would enhance cell attachment and promote cell and collagen orientation that more closely mimics native AF. The results demonstrate that the greatest cell attachment occurred with fibronectin (Fn)-coated scaffolds. Cells on Fn-coated scaffolds were also aligned parallel to scaffold fibers, a process that involved α5β1 integrin, determined by integrin-specific blocking antibodies. The inhibition of this integrin reduced AF cell spreading and alignment and the changes in cell shape were regulated by the actin cytoskeleton, demonstrated using cytochalasin D inhibitor. Cells on Fn-coated scaffolds formed fibrillar Fn, synthesized significantly more collagen, and showed alignment of type I collagen that more closely mimics native AF therefore facilitating the development of the tissue in vitro.
URI: http://hdl.handle.net/1807/27463
Appears in Collections:Master

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