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|Title: ||The Role of Cell Adhesion Genes in the Pathogenesis of Medulloblastoma|
|Authors: ||Bertrand, Kelsey C.|
|Advisor: ||Taylor, Michael|
|Department: ||Laboratory Medicine and Pathobiology|
|Issue Date: ||2-Jun-2011|
|Abstract: ||Medulloblastoma is the most common pediatric brain tumour, yet many of the underlying genetic and epigenetic factors have yet to be discovered. After a genome wide screen of a large cohort of primary medulloblastomas, we discovered that many of the genes within the cell adhesion family are affected by either copy number loss and/or decreased expression unexplained by copy number change. This led us to believe that both genetic and epigenetic factors were affecting
this gene family. Through methylation-specific PCR, RT-PCR and high-throughput methylation status analysis, we have concluded that promoter CpG methylation plays a role in the expression of the PCDH10 protein in both medulloblastoma cell lines and primary tumours. Through functional validation with a stable cell line re-expressing PCDH10, I show that cell cycle and
proliferation remain unchanged but migration is decreased in cells with PCDH10 re-expression.
This suggests that PCDH10 has characteristics of a tumour suppressor in medulloblastoma.|
|Appears in Collections:||Master|
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