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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/27598

Title: Combinatorial Microscopy of Molecular Interactions at Membrane Interfaces
Authors: Oreopoulos, John
Advisor: Yip, Christopher M.
Department: Biomedical Engineering
Keywords: atomic force microscopy
fluorescence polarization microscopy
total internal reflection fluorescence microscopy
FRET microscopy
model membranes
supported lipid bilayers
antimicrobial peptides
charge-cluster mechanism
CEACAM
membrane protein
dimerization
oligomerization
combinatorial microscopy
AFM
pTIRFM
TIRFPM
Issue Date: 13-Jun-2011
Abstract: Biological membranes are heterogeneous two-dimensional fluids composed of lipids, sterols and proteins that act as complex gateways and define the cell boundary. The functions of these interfaces are diverse and specific to individual organisms, cell types, and tissues. Membranes must take up nutrients and small molecules, release waste products, bind ligands, transmit signals, convert energy, sense the environment, maintain cell adhesion, control cell migration, and much more while forming a tight barrier around the cell. The molecular mechanisms and structural details responsible for this diverse set of functions of biological membranes are still poorly understood, however. Developing new tools capable of probing and determining the local molecular organization, structure, and dynamics of membranes and their components is critical for furthering our knowledge about these important cellular processes that are often linked to health and diseases. Combinatorial microscopy takes advantage of the rich properties of light (intensity, wavelength, polarization, etc.) to create new forms of imaging that quantify the motions, orientations, and binding kinetics of the sample’s biomolecular constituents. These new optical imaging modalities can also be further combined with other types of microscopy to produce spatially correlated micrographs that provide complementary pieces of information about the sample under investigation that would otherwise remain hidden from the observer if the two imaging techniques were applied independently. The first part of this thesis provides a detailed account of the construction of a specialized hybrid microscopy platform that combines polarized total internal reflection fluorescence microscopy (pTIRFM) with atomic force microscopy (AFM) for the purpose of studying fundamental sterol-lipid and antimicrobial peptide-lipid interactions in model membranes. The second half describes a combined pTIRFM and Förster resonance energy transfer (FRET) imaging method to elucidate the oligomeric state and spatial distribution of carcinoembryonic-antigen-related cell-adhesion molecules (CEACAMs) in the membranes of living cells.
URI: http://hdl.handle.net/1807/27598
Appears in Collections:Doctoral

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