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Medical Biophysics (MBP) >
Stem Cells >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/2775

Title: Physical separation of hemopoietic stem cells differing in their capacity for self-renewal
Authors: Worton, Ronald G., rworton_at_ohri.ca
McCulloch, Ernest A., mcculloc_at_uhnres.utoronto.ca
Till, James E., till_at_uhnres.utoronto.ca
Keywords: bone marrow cells
cell separation
CFU
colony-forming units
hematopoietic stem cells
hematopoietic progenitor cells
mouse marrow
spleen colonies
Issue Date: Jul-1969
Publisher: The Rockefeller University Press
Citation: J Exp Med 1969(Jul); 130(1): 91-103
Description: The stem cells in mouse hemopoietic tissues that may be detected by their ability to form macroscopic splenic colonies possess the capacity for self-renewal and the capacity for differentiation. They express both these properties to widely varying degrees. The possibility that colonies derived from individual stem cells differ in composition because of intrinsic differences in the stem cells themselves was tested directly. A physical technique of cell separation, velocity sedimentation, was utilized to fractionate populations of hemopoietic stem cells into subpopulations possessing different sedimentation rates. Tests were made of the capacity for self-renewal of the stem cells present in the different fractions. Stem cells from different fractions were found to renew themselves to different degrees, providing strong evidence that stochastic processes and environmental factors are not the sole sources of heterogeneity in the composition of splenic colonies. Reproduced from The Journal of Experimental Medicine, 1969(Jul); 130(1): 91-103 by copyright permission of The Rockefeller University Press (www.rockefeller.edu/rupress/).
URI: http://hdl.handle.net/1807/2775
Appears in Collections:Stem Cells
Till, James E.

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