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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/2876

Title: A validated high-performance liquid chromatography method for the determination of rifampicin and desacetyl rifampicin in plasma and urine
Authors: Kumar, A. K. Hemanth
Chandra, I.
Geetha, R.
Chelvi, K. Silambu
Lalitha, V.
Prema, G.
Keywords: Pharmacology
ph04076 Rifampin, rifamycin, HPLC
Issue Date: Aug-2004
Publisher: Medknow Publications on behalf of Indian Pharmacological Society
Citation: Indian Journal of Pharmacology 36(4)
Abstract: OBJECTIVE: To standardize a high-performance liquid chromatography (HPLC) method for the determination of rifampicin (RMP) and its major metabolite desacetyl rifampicin (DRMP) in plasma and urine. MATERIAL AND METHODS: A simple, specific and sensitive HPLC method was developed for the determination of RMP and DRMP in plasma and urine. Separation in both was achieved by reverse-phase chromatography on a C18 column with a mobile phase composition of 0.05 M phosphate buffer: acetonitrile (55:45 v/v) at 254 nm. RESULTS: The retention times of DRMP, RMP and Rifapentine (RPN), the internal standard were 2.9, 4.8 and 10.5 min respectively. The assay was linear from 0.25 to 15.0 g ml-1 for plasma and 2.5 to 80.0 g ml-1 for urine. Both intra-day and inter-day accuracy and precision data showed good reproducibility. CONCLUSION: The HPLC method described is sensitive, selective and linear for the wide range of concentrations for RMP and DRMP in plasma and urine. Thus, the method developed is well suited for the pharmacokinetic studies.
URI: http://bioline.utsc.utoronto.ca/archive/00001902/01/ph04076.pdf
Appears in Collections:Bioline International Legacy Collection

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