test Browse by Author Names Browse by Titles of Works Browse by Subjects of Works Browse by Issue Dates of Works
       

Advanced Search
Home   
 
Browse   
Communities
& Collections
  
Issue Date   
Author   
Title   
Subject   
 
Sign on to:   
Receive email
updates
  
My Account
authorized users
  
Edit Profile   
 
Help   
About T-Space   

T-Space at The University of Toronto Libraries >
School of Graduate Studies - Theses >
Master >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/29495

Title: Label-free, Direct Detection of Cocaine using an Aptamer in Conjunction with an Ultra-high Frequency Acoustic Wave Sensor
Authors: Bokhari, Syed Sumra
Advisor: Thompson, Michael
Department: Biomedical Engineering
Keywords: acoustic
biomedical engineering
cocaine
piezoelectric
biosensor
self assembled monolayers
thickness shear mode
anti-cocaine aptamer
aptasensor
Electromagnetic piezoelectric acoustic sensor
TSM
EMPAS
aptamer immobilization optimization
label-free cocaine detection
Issue Date: 11-Aug-2011
Abstract: This study embarks on exploiting the Thickness Shear Mode (TSM) acoustic wave sensor and the ElectroMagnetic Piezoelectric Acoustic Sensor (EMPAS) towards the study of aptamer-to-cocaine binding in a label-free direct approach. The high sensitivity and selectivity offered by the EMPAS in combination with alkyltrichlorosilane-based self-assembled monolayers proved superior towards the detection of cocaine. The most efficient method for the attachment of the aptamers onto the sensor surface to construct highly dense populations of the aptamer molecules with retained biomolecule activity is shown to be dependent on the composition of immobilizing solution and on the amount of spacing provided in the plane of the aptamer molecules. The distinct ligand-induced binding mechanisms and regeneration capabilities of the two anti-cocaine aptamers are monitored with the EMPAS. Utilizing this sensor to monitor cocaine-aptamer interactions will serve as the first piezoelectric aptasensor for the detection of a small molecule.
URI: http://hdl.handle.net/1807/29495
Appears in Collections:Master

Files in This Item:

File Description SizeFormat
Bokhari_Syed_S_201106_MASc_thesis.pdf1.78 MBAdobe PDF
View/Open

This item is licensed under a Creative Commons License
Creative Commons

Items in T-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

uoft