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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/29635

Title: The Influence of the Physical Environment on Annulus Fibrosus Cells Cultured on Oriented Nanofibrous Polyurethane Scaffolds
Authors: Turner, Kathleen Grace
Advisor: Kandel, Rita A.
Santerre, J. Paul
Department: Biomedical Engineering
Keywords: intervertebral disc
annulus fibrosus
tissue engineering
tension
dynamic culture
nanofibre
polyurethane scaffold
Issue Date: 25-Aug-2011
Abstract: Tissue engineering the annulus fibrosus (AF) for use in a functional intervertebral disc replacement is a promising alternative to current treatments of degenerative disc disease. Polycarbonate urethane (PU) scaffolds have demonstrated the ability to support AF cell attachment and matrix synthesis and are suitable for tissue engineering the AF. The present study investigates the effects of the physical and biochemical environment on AF cells grown on aligned nanofibrous PU scaffolds. First, the effect of dynamic spinner flask culture and fibronectin pre-coating on tissue formation was analyzed and then the role of scaffold fibre tension on annulus fibrosus cells was examined using a tailored culture system. The results of these studies demonstrated that AF cells are sensitive to differences in biochemical cues at the scaffold surface and their physical environment and respond by altering their cellular responses and, potentially by manipulating their microenvironments, including the physical characteristics of the PU-ADO scaffolds.
URI: http://hdl.handle.net/1807/29635
Appears in Collections:Master

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