test Browse by Author Names Browse by Titles of Works Browse by Subjects of Works Browse by Issue Dates of Works
       

Advanced Search
Home   
 
Browse   
Communities
& Collections
  
Issue Date   
Author   
Title   
Subject   
 
Sign on to:   
Receive email
updates
  
My Account
authorized users
  
Edit Profile   
 
Help   
About T-Space   

T-Space at The University of Toronto Libraries >
School of Graduate Studies - Theses >
Master >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/30060

Title: Characterization of Metal-binding P-loop GTPases: E. coli YeiR and M. thermoacetica AcsF
Authors: Flood, Jessica
Advisor: Zamble, Deborah
Department: Chemistry
Keywords: P-loop GTPase
metallochaperone
Issue Date: 23-Nov-2011
Abstract: Organisms express metal-binding proteins in order to deal with essential metal ions that can be potentially toxic. A common trend of bacterial metal homeostasis pathways is the presence of a GTPase, and several of these proteins are members of the G3E family of P-loop GTPases. In this work we focused on E. coli YeiR, member of the under-characterized COG0523 subfamily, and on M. thermoacetica AcsF. The in vitro metal-binding properties of isolated YeiR are presented. The protein binds Ni2+ and Zn2+ with low micromolar affinity, and oligomerizes in the presence of metal. The GTPase activity of YeiR is similar to that measured for other members of the group and is enhanced by Zn2+. In the case of AcsF, it was not possible to establish concluding evidence that the protein binds metal. This study helps shed light upon members of the under-characterized subfamily of G3E P-loop GTPases.
URI: http://hdl.handle.net/1807/30060
Appears in Collections:Master

Files in This Item:

File Description SizeFormat
Flood_Jessica_A_201111_MSc_thesis.pdf3.1 MBAdobe PDF
View/Open

Items in T-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

uoft