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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/3011

Title: The cellular basis of the genetically determined hemopoietic defect in anemic mice of genotype Sl/Sld
Authors: McCulloch, Ernest A., mcculloc_at_uhnres.utoronto.ca
Siminovitch, Louis, lsiminovitch_at_mtsinai.on.ca
Till, James E., till_at_uhnres.utoronto.ca
Russell, Elizabeth S.
Bernstein, Seldon E.
Keywords: assay for progenitor cells
colony-forming units
hematopoietic progenitor cells
hematopoietic stem cells
hematopoietic stem cell transplantation
inbred mouse strains
mouse marrow
mouse spleen
spleen colonies
Issue Date: 1-Oct-1965
Publisher: American Society of Hematology
Citation: Blood 1965(1 Oct) 26(4): 399-410
Abstract: Summary: The proliferation and function of hemopoietic cells derived from genetically anemic Sl/Sld mice have been studied by the use of cell transplantation technics. It was found that marrow cells derived from anemic Sl/Sld or Sld/Sld mice, when implanted into heavily irradiated mice of genotype +sl/+sl, are capable of forming macroscopic spleen colonies, with approximately the same frequency as cells derived from normal +sl/+sl mice. Marrow cells derived from Sl/Sld animals were tested for their capacity to cure the anemia of W/Wv mice and were found to implant as rapidly and to have as long-lasting a beneficial effect as did marrow cells from +sl/+sl mice. The radiosensitivity of the colony-forming ability of marrow cells from Sl/Sld mice was found to be similar to that found previously for +sl/+sl marrow cells, although the anemic animals are known to be more sensitive to total-body irradiation than are their normal littermates. Marrow cells from +sl/+sl mice were found to proliferate more slowly in irradiated mice of genotype Sl/Sld than in irradiated +sl/+sl littermates, even when the anemic and the normal mice were joined in parabiosis. These observations indicate that the hemopoietic colony-forming cells in mice of genotype Sl/Sld are normal, but fail to function adequately because the tissues of mice of this genotype are unable to provide sufficient support for proliferation and differentiation of these progenitor cells.
Description: Reproduced by copyright permission of the American Society of Hematology (www.hematology.org). All of Blood's back content becomes free to anybody in the world, whether or not associated with a subscribing library, as soon as it is 12 months old (see www.hematology.org/government/policy/open_access.cfm).
URI: http://hdl.handle.net/1807/3011
Appears in Collections:Stem Cells
Till, James E.

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