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|Title: ||The cellular basis of the genetically determined hemopoietic defect in anemic mice of genotype Sl/Sld|
|Authors: ||McCulloch, Ernest A., mcculloc_at_uhnres.utoronto.ca|
Siminovitch, Louis, lsiminovitch_at_mtsinai.on.ca
Till, James E., till_at_uhnres.utoronto.ca
Russell, Elizabeth S.
Bernstein, Seldon E.
|Keywords: ||assay for progenitor cells|
hematopoietic progenitor cells
hematopoietic stem cells
hematopoietic stem cell transplantation
inbred mouse strains
|Issue Date: ||1-Oct-1965|
|Publisher: ||American Society of Hematology|
|Citation: ||Blood 1965(1 Oct) 26(4): 399-410|
|Abstract: ||Summary: The proliferation and function of hemopoietic cells derived from genetically anemic Sl/Sld mice have been studied by the use of cell transplantation technics. It was found that marrow cells derived from anemic Sl/Sld or Sld/Sld mice, when implanted into heavily irradiated mice of genotype +sl/+sl, are capable of forming macroscopic spleen colonies, with approximately the same frequency as cells derived from normal +sl/+sl mice. Marrow cells derived from Sl/Sld animals were tested for their capacity to cure the anemia of W/Wv mice and were found to implant as rapidly and to have as long-lasting a beneficial effect as did marrow cells from +sl/+sl mice. The radiosensitivity of the colony-forming ability of marrow cells from Sl/Sld mice was found to be similar to that found previously for +sl/+sl marrow cells, although the anemic animals are known to be more sensitive to total-body irradiation than are their normal littermates. Marrow cells from +sl/+sl mice were found to proliferate more slowly in irradiated mice of genotype Sl/Sld than in irradiated +sl/+sl littermates, even when the anemic and the normal mice were joined in parabiosis. These observations indicate that the hemopoietic colony-forming cells in mice of genotype Sl/Sld are normal, but fail to function adequately because the tissues of mice of this genotype are unable to provide sufficient support for proliferation and differentiation of these progenitor cells.|
|Description: ||Reproduced by copyright permission of the American Society of Hematology (www.hematology.org). All of Blood's back content becomes free to anybody in the world, whether or not associated with a subscribing library, as soon as it is 12 months old (see www.hematology.org/government/policy/open_access.cfm).|
|Appears in Collections:||Stem Cells|
Till, James E.
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