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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/30589

Title: Calpain and Calpastatin in a Mouse Model of Acute Myeloid Leukemia
Authors: Farr, Christina
Advisor: Berger, Stuart
Department: Immunology
Keywords: calpain
acute myeloid leukemia
Issue Date: 7-Dec-2011
Abstract: I have studied the calpain system in acute myeloid leukemia using the 32D and 32Dkit cell lines. Specifically, I characterized the calpain system in the cell lines, and performed calpastatin overexpression and knockdown studies. I found that calpain activity is elevated in the 32D and 32Dkit cells, and calpain inhibition causes apoptosis. Both μ- and m-calpain contribute to the calpain activity in these cell lines. The 32Dkit cells have higher calpain activity than the 32D cells, which I have shown is partially attributed to basal ckit activation. Calpastatin was present in both cell lines, but exists mainly in a degraded form. Calpastatin overexpression lowered calpain activity and provided a growth disadvantage to the 32Dkit cells, but had no effect on 32D cells. Calpastatin knockdown caused a significant increase in calpain activity in the 32D cells, which changed the cell cycle distribution but had no other major effects.
URI: http://hdl.handle.net/1807/30589
Appears in Collections:Master

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