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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/31398

Title: Generation of Planar Cell Polarity (PCP) in vitro for Epithelium Tissue Engineering
Authors: Paz Mejia, Ana Cristina
Advisor: McGuigan, Alison P.
Department: Chemical Engineering and Applied Chemistry
Keywords: Epithelium Tissue Engineering
Planar Cell Polarity
Issue Date: 19-Dec-2011
Abstract: Engineering epithelium with correct structure is essential for generating functional tissue. During tissue development, cells organize in defined patterns through cellular signalling. Artificial generation of the signalling that organizes cells within the tissue offers a novel approach for engineering tissues with appropriate structure. Planar cell polarity (PCP) is a cellular signalling pathway involved in the organization of epithelial cells. Our goal is to study the effect that co-culturing genetically distinct populations of epithelial cells, with variable levels of one of the core PCP proteins, has in epithelial cell sheet organization. MDCK cells transduced with a tagged PCP core protein (GFP-Vangl2) and wild type MDCK cells were co-cultured side-by-side. The effect of tight junction and cilia formation, and localization of the GFP-Vangl2 protein were evaluated. The results suggest that tight junction and cilia formation are not affected. On the other hand, the GFP-Vangl2 protein seems to be affected at some level.
URI: http://hdl.handle.net/1807/31398
Appears in Collections:Master

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