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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/31618

Title: Blockade of TLR2 Inhibits P. gingivalis Suppression of Mineralized Matrix Formation by Human Dental Pulp Stem Cells
Authors: Tom-Kun Yamagishi, Valerie
Advisor: Glogauer, Michael
Department: Dentistry
Keywords: DPSC
Pulp Regeneration
Issue Date: 2-Jan-2012
Abstract: In an effort to re-establish tissue with odontogenic potential in the pulp space of immature permanent teeth, stimulated human dental pulp stem/progenitor cells (hDPSCs) have shown potential to differentiate and form mineralized matrix, marked by high expression of dentin sialophosphoprotein (DSPP) and osteocalcin (OCN). Bacterial by-products have been shown to adversely affect cell differentiation. This study investigated the effect of P. gingivalis, a putative endodontic pathogen, and blockage of its host recognition on hDPSCs. Stimulated hDPSCs were exposed to varying concentrations of P. gingivalis by-product and gene expression of DSPP and OCN was measured. Cells were exposed to TLR2 blocking agents prior to exposure to the by-product. P. gingivalis affected dose-dependent suppression of the measured gene expression. Blockade of TLR2 inhibited the by-product derived suppression of gene expression. The immune-potential of by-product was confirmed to be detrimental to the differentiation of hDPSCs, and this effect could be moderated by TLR2-blockade.
URI: http://hdl.handle.net/1807/31618
Appears in Collections:Master

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