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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/32231

Title: Mutation and Loss of Heterozygosity in an Individual of the Root-infecting Fungus Armillaria Gallica in a Mixed Hardwood Forest
Authors: Catona, Stefan
Advisor: Anderson, James
Department: Ecology and Evolutionary Biology
Keywords: Armillaria gallica
somatic mutation
Issue Date: 21-Mar-2012
Abstract: Long-lived individuals of the opportunistic fungal pathogen Armillaria gallica arise in single mating events, and then grow vegetatively to occupy large territories including multiple woody substrates. In effect, this leaves a spatial record of mutation, the detection of which would allow new inferences about how fungal individuals grow and infect their hosts. In this thesis, I first identified a large individual of A. gallica in eastern Ontario. I then searched for genetic variation within this individual by focusing on the tandemly repeated rRNA gene cluster and four microsatellite markers that are variable in the A. gallica population. I discovered a loss of heterozygosity (LOH) in the rRNA gene-cluster region, forming two genotypes that show significant spatial clustering in a Mantel test. My M.Sc. thesis research serves as a baseline for a genome-wide study of the mutational dynamic within the vegetative growth phase of this large and old Armillaria individual.
URI: http://hdl.handle.net/1807/32231
Appears in Collections:Master

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