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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/32468

Title: Validation of de novo Bioinformatic Predictions of Arabidopsis thaliana Cis-regulatory Elements using in planta GUS Expression Assays
Authors: Hiu, Shuxian
Advisor: Provart, Nicholas
Department: Cell and Systems Biology
Keywords: cis-regulatory elements
bioinformatics
GUS
cold stress
enhancer
silencer
insulator
local control regions (LCRs)
MARs/SARs
in planta
evening element
circadian rhythm
arabidopsis
genotoxic
bleomycin
mitomycin c
heat stress
stem epidermis
root
abscisic acid (ABA)
osmotic stress
salt stress
synthetic constructs
expression assays
transcription factor binding sites
CaMV35S
columbia-0
Issue Date: 19-Jul-2012
Abstract: The study of cis-regulatory elements (CREs) will allow for increased understanding of regulation and lead to insight regarding the mechanisms governing growth, development, health, and disease. The aim of this study was to characterize the de novo in silico predictions of Arabidopsis CREs. Eight synthetic and 30 native promoter-constructs containing an eGFP/GUS reporter protein were generated for cold, genotoxic, heat, osmotic, and salt stress; the circadian clock; ABA signaling; root and epidermis tissue. Constructs were stably transformed into A. thaliana Col-0 and the effects of the CREs were evaluated by in planta stress or tissue assays using GUS expression levels. Results reveal a novel genotoxic element that specifically directs GUS expression in rosette leaves during genotoxic stress. Results also look promising for novel epidermis and root-specific elements. Results of these assays validate the de novo prediction pipeline's ability to identify novel and known CREs related to abiotic stress.
URI: http://hdl.handle.net/1807/32468
Appears in Collections:Master

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