test Browse by Author Names Browse by Titles of Works Browse by Subjects of Works Browse by Issue Dates of Works
       

Advanced Search
Home   
 
Browse   
Communities
& Collections
  
Issue Date   
Author   
Title   
Subject   
 
Sign on to:   
Receive email
updates
  
My Account
authorized users
  
Edit Profile   
 
Help   
About T-Space   

T-Space at The University of Toronto Libraries >
School of Graduate Studies - Theses >
Master >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/33217

Title: Bacteria Filamentation, a Contributing Factor to the Intracellular Survival of Legionella pneumophila
Authors: Gigliozzi, Darren
Advisor: Terebiznik, Mauricio
Department: Cell and Systems Biology
Keywords: Legionella
Macrophages
Issue Date: 20-Nov-2012
Abstract: Legionella pneumophila (Lp) is the pathogen responsible for Legionnaires disease. Lp invades and survives in human macrophages to form an intracellular compartment, called the Legionella containing vacuole (LCV). Within the LCV, Lp avoids degradation and replicates, before killing its host and releasing its virulent progeny. Lp is pleomorphic, exhibiting a combination of short rod phenotypes and filaments. Filamentous Lp has been reported in patient samples, but current studies of Lp virulence are exclusively focused on rods. Our results show that filamentous Lp can invade and replicate in human and murine macrophages. Filaments are phagocytosed gradually into tubular phagocytic cups. Interestingly, the formation of the LCV starts at this stage of phagocytosis, and LCV markers were detected before the sealing of the phagosome occurred. We present evidence that the filamentous morphology acts cooperatively with Lp effectors to subvert the microbicidal activities of the macrophage, contributing to the survival of Lp.
URI: http://hdl.handle.net/1807/33217
Appears in Collections:Master

Files in This Item:

File Description SizeFormat
Gigliozzi_Darren_C_2012_MSc_thesis.pdfThesis222.18 MBAdobe PDF
View/Open
Gigliozzi_Darren_C_2012_MSc_Movie S1.movMovie2.37 MBVideo Quicktime
View/Open
Gigliozzi_Darren_C_2012_MSc_Movie S2.movMovie1.28 MBVideo Quicktime
View/Open
Gigliozzi_Darren_C_2012_MSc_Movie S3.movMovie1.1 MBVideo Quicktime
View/Open
Gigliozzi_Darren_C_2012_MSc_Movie S4.movMovie1.75 MBVideo Quicktime
View/Open
Gigliozzi_Darren_C_2012_MSc_Movie S5.movMovie1.15 MBVideo Quicktime
View/Open
Gigliozzi_Darren_C_2012_MSc_Movie S6.movMovie6.16 MBVideo Quicktime
View/Open
Gigliozzi_Darren_C_2012_MSc_Movie S7.movMovie1.07 MBVideo Quicktime
View/Open
Gigliozzi_Darren_C_2012_MSc_Movie S8.movMovie979.58 kBVideo Quicktime
View/Open

Items in T-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

uoft