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Please use this identifier to cite or link to this item: http://hdl.handle.net/1807/33242

Title: Towards a Novel Electrochemical Sensing Platform for Diagnosing Urinary Tract Infections
Authors: Holmes, Richard
Advisor: Kelley, Shana O.
Department: Biomedical Engineering
Keywords: electrochemical sensing
antimicrobial resistance
bacterial detection
nanostructured microelectrodes
urinary tract infection
surface functionalization
Issue Date: 20-Nov-2012
Abstract: Urine culture, the current gold standard for urinary tract infection (UTI) diagnosis, does not produce results in an acceptable length of time. An ultra-sensitive, cost-effective electrochemical biosensing platform with nanostructured microelectrodes was designed to address the need for a rapid, point-of-care (PoC) test that could achieve a sample-to-answer time in less than an hour. Printed circuit boards and metallized glass slides were processed using various techniques and then tested for their ability to form nanostructured microelectrodes. Peptide nucleic acid probes for the bacteria and yeast as well as ten probes for antibiotic resistance genes were designed and synthesized for use with the new platform. Validation of the sensor's specificity was performed using high concentrations (100nM) of synthetic DNA oligomers. Furthermore, a clinically relevant sensitivity of 103 cfu/mL was demonstrated by detecting 4 pathogen lysates (Staphylococcus saprophyticus, Pseudomonas aeruginosa, Enterococcus faecalis and Klebsiella pneumoniae) in a buffered solution.
URI: http://hdl.handle.net/1807/33242
Appears in Collections:Master

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